Fluid Pack Test Procedure

The individual tests described below (Set-up, Purge, WISP™ Pressure Test, etc.) are meant to be done in the sequence shown. If the sequence is not followed, the results may be inaccurate.

Definitions:

1. RAD pin 'up' means 'open'; 'down' means 'closed'.
2. A WISP™ reset means RAD pins 1 and 8 are up, all others are down. This is equivalent to pushing the 'RESET' button on the front panel.

Set-up

1. Ramp test your pump to insure that it will hold up to 5000 psi without losing pressure.
2. Reset the WISP™ (RAD pins 1 and 8 up, all others down).
3. Remove the carriage.
4. Connect the WISP™ blue line (.040 ID) to the pump transducer outlet.

Purge

1. Cap WISP™ red line coming from the injector head.
2. Lift (pull upward) RAD pins 3 up, 2 up and 1 up in that order. (NOTE: The order is important because it protects the syringe from being overpressured.)
3. Set flow to 4 ml/min.
4. Push RAD pin 1 down; (This back flushes the Fluid Pack). Flow goes from V1 up through the injector to V2, V3 and out the clear line to waste.
5. Remove the knurled nut on glass syringe's plunger and cycle plunger up and down several times to remove air at the plunger tip. Replace knurled nut.
6. Set flow rate to zero.

WISP™ Pressure Test

1. Reset WISP™. (RAD pins 1 and 8 up, all others down)
2. Ramp WISP™ to 4500 psi at 1 ml/min. flow rate. Pressure should hold at 4500 for 15 seconds. A decay rate of less than 500 psi in 15 seconds is acceptable.
3. If pressure does not hold, check all compression screw fittings for possible slow leakage, then snug the leaking fitting(s)
4. Set flow rate to zero.

Injector Needle Speed Test

1. Reset WISP™. (RAD pins 1 and 8 up, all others down)
2. Remove the carriage so the injector needle will not get bent.
3. Set RAD pins 6 up and 8 down. Needle should extend in the carriage compartment with the needle tip 1 cm above the base plate.
4. Reverse RAD pins 6 and 8 (6 down, 8 up).

   NOTE: The needle should descend or rise in about 2 seconds. If not, set air pressure to 30 psi. If air pressure is already at 30 psi and the problem still occurs the injector should be rebuilt. The seals are binding too much for proper operation.

5. Repeat the test several times while watching the needle movement.

Injector Compression Seal Test

1. Reset WISP™. (RAD pins 1 and 8 up, all others down)
2. Set RAD pin 3 up, 6 up and 8 down. (The needle will enter the compartment.)
3. Set RAD pins 7 up, 6 down and 8 up. (Returns needle port into lower injector seal)
4. Set pump pressure limit to 3000 psi and flow to 1 ml/min.

   NOTE: This pressure is good for testing 710B Injectors. For 712 Injectors the pressure limit should be 1000 psi and the seal should hold at least 500 psi in order to pass the test.

5. Pressure should hold at 2500 psi for 15 seconds.
6. Set RAD pin 2 up (releases pressure to clear waste line).
7. Repeat the test several times to verify seal performance.

Test HPV #1 (V1)

1. Reset WISP™. (RAD pins 1 and 8 up, all others down)
2. Set RAD pins 6 up, 8 down (needle enters carriage compartment)
3. Set Pressure Limit to 4500 psi. Set flow to 1 ml/min. Solvent should flow freely from the needle port, if pump pressure rises, the needle port is blocked. (unheard of occurrence)
4. Set RAD pin 1 down (closing valve 1 to build pressure).
5. Set RAD pin 1 up (opening V1 to release pressure). Pressure should drop to zero in 1 second - 2 to 3 seconds is too long.
6. Repeat steps 4 and 5 several times to verify valve operation.
7. Set pump flow to zero.

Test HPV #2 (V2)

1. Reset WISP™. (RAD pins 1 and 8 up, all others down)
2. Set RAD pin 3 up (prevents mistaken overpressuring of syringe)
3. Set pump pressure limit to 4500 psi. Set flow to 1 ml/min.
4. Set RAD pin 2 up. Pressure should drop to zero in 1 second.
5. Repeat the test several more times.
6. Set pump flow to zero.

Test LPV #3 (V3)

1. Reset WISP™. (RAD pins 1 and 8 up, all others down)
2. Set Pump pressure meter switch to 0 to 600 psi range.
3. Set pressure limit to 500 psi approximately.
4. Set RAD pins 3 up, 2 up in that order. (NOTE: The order is important because it protects the syringe from being overpressured.)
5. Set pump flow to 1 ml/min. (Flow should come out the clear waste line.)
6. Set RAD pin 3 down (pressure should hold at 400 psi for 15 seconds).
7. Set RAD pin 3 up (pressure should drop to zero in 1 second).
8. Repeat the test several more times.
9. Set pump flow to zero.

NOTE: This test is valid for both 710 and 712 autosamplers. If this test fails, the valve needs to be rebuilt.

If you need any further assistance, please feel free to call 952-895-8292 or toll-free at 800-670-4667 during the hours of 8:00 AM - 5:00 PM Central Time. Thanks for calling!